| 酒酒球菌中生物胺相关基因的检测 |
| |
| 引用本文： | 赵艳卓,刘树文. 酒酒球菌中生物胺相关基因的检测[J]. 中国酿造, 2011, 0(12) |
| |
| 作者姓名： | 赵艳卓 刘树文 |
| |
| 作者单位： | 1. 西北农林科技大学葡萄酒学院,陕西杨凌,712100
2. 西北农林科技大学葡萄酒学院,陕西杨凌712100;陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100 |
| |
| 基金项目： | 现代农业产业技术体系建设专项资金资助项目(nycytx-30-ch-03); 农业部“948”项目(2009Z29) |
| |
| 摘 要： | 酒酒球菌在葡萄酒苹果酸-乳酸发酵过程中可通过脱羧基作用将氨基酸转化为生物胺.该研究采用PCR技术对27株酒酒球菌中与生物胺产生相关的基因-组氨酸脱羧酶基因、鸟氨酸脱羧酶基因、酪氨酸脱羧酶基因进行了检测,研究了这些菌株产生生物胺的特性.结果显示,所有菌株均不含有组氨酸脱羧酶基因、鸟氨酸脱羧酶基因、酪氨酸脱羧酶基因,不具有产生组胺、腐胺和酪胺的能力,因而具有较高的生物胺代谢安全性.
|
| 关 键 词： | 酒酒球菌 组氨酸脱羧酶基因 酪氨酸脱羧酶基因 鸟氨酸脱羧酶 聚合酶链反应 |
Detection of biogenic amines relative genes in Oenococcus oeni |
| |
| ZHAO Yanzhuo , LIU Shuwen. Detection of biogenic amines relative genes in Oenococcus oeni[J]. China Brewing, 2011, 0(12) |
| |
| Authors: | ZHAO Yanzhuo LIU Shuwen |
| |
| Affiliation: | ZHAO Yanzhuo1,LIU Shuwen1,2(1.College of Enology,Northwest A&F University,Yangling 712100,China,2.Shaanxi Engineering Research Center for Viti-Viniculture,China) |
| |
| Abstract: | Biogenic amines could be formed from amino acids through decarboxylation by the Oenococcus oeni in malolactic fermentation.Biogenic amines related genes which were histidine decarboxylase(hdc)gene,ornithine decarboxylase(odc)gene,tyramine decarboxylase(tdc)gene in 27 Oenococcus oeni strains were detected with PCR technology.The results showed that all the strains did not harbor the hdc,odc,tdc genes,and without ability to produce histidine,putrescine and tyramine.The strains are highly safety in metabolism ... |
| |
| Keywords: | Oenococcus oeni hdc gene tdc gene odc gene polymerase chain reaction(PCR) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
