| Zn2+对制麦中苹果酸脱氢酶和呼吸消耗的影响 |
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| 引用本文： | 侯韶伟,王越,安家彦.Zn2+对制麦中苹果酸脱氢酶和呼吸消耗的影响[J].中国酿造,2011(12). |
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| 作者姓名： | 侯韶伟 王越 安家彦 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁大连,116034 |
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| 摘 要： | 研究了在制麦过程中添加Zn2+对苹果酸脱氢酶(MDH)活性和大麦呼吸消耗的影响.结果表明在制麦过程中添加浓度为0.50mmol/L、0.75mmol/L、1.00mmol/L的Zn2+均可有效的抑制MDH活力,进行动力学分析得出Zn2+大麦MDH的抑制类型为非竞争性抑制；添加浓度为0.75mmol/L、1.00mmol/L的Zn2可降低制麦过程中的呼吸消耗,其中添加1.00mmol/L Zn2+的试验组的呼吸消耗率最低,相对于对照组,两种大麦的呼吸消耗分别降低了5.22％和10.7％.同时研究了Zn2对于麦芽中的α、β淀粉酶和果胶酶活力影响,表明对α、β淀粉酶有促进作用,对果胶酶影响不明显.
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| 关 键 词： | 锌离子 苹果酸脱氢酶 制麦 呼吸消耗 |
Effect of Zn~(2+) on the activity of malate dehydrogenase and respiration consumption in the process of malting |
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| HOU Shaowei
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WANG Yue
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AN Jiayan.Effect of Zn~(2+) on the activity of malate dehydrogenase and respiration consumption in the process of malting[J].China Brewing,2011(12). |
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| Authors: | HOU Shaowei WANG Yue AN Jiayan |
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| Affiliation: | HOU Shaowei,WANG Yue,AN Jiayan(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | The effect of different Zn2+ concentration on the activity of malate dehydrogenase(MDH) and respiration consumption in the process of malting were determined.The results showed that the activity of MDH was inhibited by Zn2+,and the inhibition was noncompetitive type.The respiration consumption was reduced in the process of malting when Zn2+ concentration was 0.75mmol/L and 1.00mmol/L.The treatment group of 1.00mmol/L Zn2+ had the lowest respiration consumption compared with those of other group.Compared to ... |
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| Keywords: | Zn2 malate dehydrogenase malting respiration consumption |
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