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酱油生产中应用米曲霉和黑曲霉混合制曲的探索
引用本文:李保英,姜佳丽,蒋予箭.酱油生产中应用米曲霉和黑曲霉混合制曲的探索[J].中国酿造,2011(12).
作者姓名:李保英  姜佳丽  蒋予箭
作者单位:浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室,浙江杭州310012
基金项目:浙江省成果转化资金项目(2011D70044)
摘    要:该文考察了米曲霉和黑曲霉在单独制曲及混合制曲条件下,成曲的中性蛋白酶、酸性蛋白酶、糖化酶及孢子数,探讨了酱油多菌种制曲的可行性.实验以豆粕、麸皮为原料,以AS3.042米曲霉、AS3.350黑曲霉为菌种,探讨了2种制曲方式及不同配比制曲效果的影响.结果表明,米、黑曲霉分开制曲比混合接种制曲所得成曲效果好,米、黑曲霉分别以33℃制曲42h、48h得成品曲,再以2∶1混合所得成曲可获得较佳的酶活力,曲料总蛋白酶活力2748U/g与对照值相当,而酸性蛋白酶及糖化酶活力分别达712U/g、1090U/g,较对照分别提高55.8%、25.4%.

关 键 词:双菌种制曲  分开制曲  混合制曲

Application of a mixed koji-making system by Aspergillus oryzae and Aspergillus niger in soy sauce production
LI Baoying , JIANG Jiali , JIANG Yujian.Application of a mixed koji-making system by Aspergillus oryzae and Aspergillus niger in soy sauce production[J].China Brewing,2011(12).
Authors:LI Baoying  JIANG Jiali  JIANG Yujian
Affiliation:LI Baoying,JIANG Jiali,JIANG Yujian(College of Food Science and Biotechnology,Zhejiang Key Laboratory of Food Safe,Zhejiang Gongshang University,Hangzhou 310012,China)
Abstract:The neutral protease activity,acid protease activity,glucoamylase activity and the number of spores in separate koji-making and mixed koji-making by Aspergillus oryzae and Aspergillus niger were measured in this paper.The feasibility of multi-strains for soy sauce koji was discussed.The effects of using AS3.042 and AS3.350 as fermented culture on koji-making were investigated.The results showed that separate koji-making was better than mixed koji-making in all respects.The optimal koji-making conditions wer...
Keywords:two molds koji-making  separated koji-making  mixed koji-making  
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