氧化还原电位控制的自絮凝酵母自动重复批次发酵

引言随着石油资源的日益减少和环境污染的逐渐加剧,使用可再生的清洁能源已经是世界各国的共识。在众多形式的非矿物质能源中,基于生物质的燃料乙醇已得到了广泛的应用1]。但是,由于生产成本较高,各国的燃料乙醇生产大都依靠着政策扶植和税收优惠2]。因此,看似十分"成熟"的乙醇生产产业,仍然需要进一步开发降低成本的创新技术3]。近些年来,通过基因工程手段改造菌种4]、

Automatic consecutive batch ethanol fermentation with self-flocculating yeast under ORP control

Consecutive batch fermentation with self-flocculating yeast realized recycling of cell and increased ethanol yield and productivity.Oxidoreduction potential(ORP)control was used in this process to automatically feed the fresh medium at the end of every batch by judging the uprising of ORP curve.When ORP value was controlled at-100 mV and initial glucose were chosen as 200 g·L^-1(industrial concentration)and 250 g·L^-1(very high gravity),compared with batch fermentation,accumulated biomass in consecutive batch sharply declined by 42.4%(～200 g·L^-1,5 batches)and 16.3%(～250 g·L^-1,6 batches),respectively.On the other hand,compared with manual consecutive batch fermentation,automatic process greatly shortened fermentation time by 17.8%(～200 g·L^-1)and 15.2%(～250 g·L^-1).Consequently,ethanol productivities were enhanced by 21.7% and 17.9%,respectively.