超高效液相色谱-串联质谱法测定瘦猪肉中的克伦特罗

建立了超高效液相色谱-串联质谱/质谱法测定瘦猪肉中克伦特罗的方法。试样用正己烷脱脂,10%亚铁氰化钾及20%乙酸锌作为沉淀剂,以乙酸乙酯提取,采用电喷雾离子源,正离子扫描,多反应监测(MRM)模式进行检测,外标法定量。结果表明,克伦特罗在1~100μg/L浓度范围内线性关系良好,空白样品加标回收率均大于85%,相对标准偏差小于5%。克伦特罗方法检出限(LOD)均为0.5μg/kg。该方法准确、灵敏、操作相对简便,有效降低基质干扰,适用于瘦猪肉中克伦特罗的测定。

Determination of Clenbuterol in Lean pork by UPLC-MS/MS

An ultraperfbrmance liquid chromatography-tandemmassspectrometry （UPLC-MS/MS） method was established for the determination of clenbuterol in lean pork.The Samples were processed with 10 % and 20 % of potassium ferrocyanide zinc acetate as the precipitating agent to reduce matrix interference, defatted with hexane, extracted with ethyl acetate and then detected by UPLC-MS/MS under electrospray ionization, positive ion scan, multiple reaction monitoring （MRM） mode, fixed quantity with external standard. The results showed that clenbuterol at 1-100 μg/L concentration ranged a good linear relationship, blank samples spiked recoveries were greater than 85 %, the relative standard deviation was less than 5 %. Clenbuterol method detection limits （LOD） were all 0.5 μg/kg. The method was accurate, sensitive, and the operation was relatively simple and effective to reduce matrix interference, suitable for determination of clenbuterol in lean pork.