反胶团酶反应过程开发研究进展

反胶团酶反应具有水相与有机相酶反应的共同优点 ,因而受到人们的重视。制约其实际应用的重要原因在于如何解决酶的重复利用问题。最初人们采用水溶液萃取法回收酶 ,但由于条件苛刻 ,酶活回收率较低。相变法利用温度的改变使体系分相以实现酶的回收 ,操作简便 ,但仍有较大的酶活损失。连续流两相离心反应器可以实现反胶团酶反应的连续操作且酶的损失很小 ,但该反应器传质速率较低因而转化率不高。膜反应器是反胶团酶反应中研究最多也最有应用前途的一种反应器形式。文中介绍了反胶团酶反应中酶重复利用的方法 ,对其过程开发的最新进展进行了综述。

Progress of Process Development of Enzymatic Reactions in Reversed Micelles

Enzymatic reactions in reversed micelles were received much attention for its combination of the advantages of enzymatic reactions in aqueous solution and in organic solvent.One important problem which restricts the application of this technique was the reuse of the enzyme.Originally the enzyme was extracted from the reversed micelles by aqueous solution.The remaining of the enzyme activity was low because of the severe conditions.The enzyme can also be reused by phase separation caused by changes in the temperature.This method was manageable,but the remaining of the enzyme activity was also low.The continuous enzymatic reactions in reversed micelles became feasible by using continuous flow two phase centrifugal reactor.But the conversion in the reactor was limited by the lower transfer rate of substrate and product.Membrane reactor was mostly studied and was looked as the most promising reactor for enzymatic reaction in reversed micelles.The methods of enzyme reuse of enzymatic reactions in reversed micelles were introduced in this article.The progress of process development of enzymatic reactions in reversed micelles was reviewed.