Chromatographic separation of glucopyranosyl sinapate from canola meal |
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| Authors: | Ryszard Amarowicz Fereidoon Shahidi |
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| Affiliation: | (1) Department of Biochemistry, Memorial University of Newfoundland, St. John’s, A1B 3X9, NF, Canada;(2) Department of Chemistry, Memorial University of Newfoundland, St. John’s, A1B 3X9, NF, Canada;(3) Department of Food Science, Centre for Agrotechnology and Veterinary Science, Polish Academy of Sciences, Olsztyn, Poland |
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| Abstract: | The compound 1-O-β-D glucopyranosyl sinapate (GPS), a phenolic glycoside, was separated from ethanolic extracts of defatted canola meal by a two-step chromatographic method. The first step involved Sephadex LH-20 chromatography with methanol as the eluting solvent. The solvent from the fraction containing GPS was evaporated, and glucopyranosyl sinapate was subsequently separated by a semi-preparative high-performance liquid chromatography method with an RP-18 column and a mobile phase consisting of water/acetonitrile/acetic acid (88∶10∶2, vol/vol/vol). |
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| Keywords: | Antioxidant activity canola meal glucopyranosyl sinapate HPLC phenolics Sephadex LH-20 chromatography |
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