Adipose hormone-sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides |
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Authors: | Victor C Gavino Grace R Gavino |
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Affiliation: | (1) Department of Nutrition, Université de Montréal, Station A, P.O. Box 6128, H3C 3J7 Montréal, Québec, Canada |
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Abstract: | Rat adipose hormone-sensitive lipase-mediated release of fatty acids from triglycerides was studied in three model systems:
i) cultured preadipocytes containing polyunsaturated fatty acid-enriched triglyceride; ii) perfused epididymal fat pads; and
iii)in vitro incubations of crude preparations of hormone-sensitive lipase with synthetic triglyceride-analogues as substrates. We found
that cultured preadipocytes challenged with 10μM norepinephrine tended to release more ω6 and ω3 polyunsaturated fatty acids
than saturated fatty acids. Fat pads perfused with 10 μM norepinephrine preferentially released arachidonate and α-linolenate
but tended to retain oleate and linoleate. Finally, crude preparations of hormonesensitive lipase released from the triglyceride-analogue
substrates α-linolenate twice as fast as oleate. We conclude that rat adipose hormone-sensitive lipase preferentially releases
polyunsaturated fatty acids from triglycerides. We suggest that this may be a mechanism by which these fatty acids are kept
from being trapped in fat depots and maintained in the circulation. |
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