| 柱层析法分离纯化大豆磷脂酰胆碱 |
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| 引用本文： | 韩雪,汪勇,胡长鹰,王兴国,刘元法. 柱层析法分离纯化大豆磷脂酰胆碱[J]. 食品研究与开发, 2010, 31(3) |
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| 作者姓名： | 韩雪 汪勇 胡长鹰 王兴国 刘元法 |
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| 作者单位： | 1. 暨南大学,食品科学与工程系,广东,广州,510632
2. 江南大学,食品学院,江苏,无锡,214122 |
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| 摘 要： | 利用硅胶柱层析法,通过梯度洗脱对大豆粉末磷脂中的磷脂酰胆碱进行分离纯化.比较氯仿/甲醇和异丙醇/甲醇两个洗脱体系所得磷脂酰胆碱的纯度和回收率.梯度洗脱时,在氯仿/甲醇体系中氯仿:甲醇的比例为3:2(体积分数),异丙醇/甲醇体系异丙醇:甲醇的比例为1:1(体积分数)洗脱,是获得磷脂酰胆碱的最佳洗脱条件.结果表明:在氯仿,甲醇洗脱体系获得磷脂酰胆碱的纯度为97.0%、回收率为86.5%.异丙醇/甲醇洗脱体系获得磷脂酰胆碱的纯度为95.0%、回收率为83.0%.
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| 关 键 词： | 磷脂酰胆碱 硅胶 柱层析 纯化 |
Separation and Purification the Phosphatidylcholine from Soy Lecithin Powder by Column Chromatography |
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| HAN Xue,WANG Yong,HU Chang-ying,WANG Xing-guo,LIU Yuan-fa. Separation and Purification the Phosphatidylcholine from Soy Lecithin Powder by Column Chromatography[J]. Food Research and Developent, 2010, 31(3) |
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| Authors: | HAN Xue WANG Yong HU Chang-ying WANG Xing-guo LIU Yuan-fa |
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| Affiliation: | HAN Xue1,WANG Yong1,HU Chang-ying1,WANG Xing-guo2,LIU Yuan-fa2 (1.Department of Food Science , Engineering,Jinan University,Guangzhou 510632,Guangdong,China,2.College of Food Science,Jiangnan University,Wuxi 214122,Jiangsu,China) |
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| Abstract: | Separation and purification the phosphatidylcholine(PC)from soy lecithin powder by gradient elution using the silica gel column chromatography was stduied.Purity and recovery of phosphatidylcholine,which obtained from chloroform/methanol and isopropyl alcohol/methanol elution system were compared.The optimal conditions for elution of phosphatidylcholine were chloroform/methanol (3:2)and isopropyl alcohol/methanol (1:1)for chloroform/methanol and isopropyl alcohol/methanol system,respectively.The purities of... |
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| Keywords: | phosphatidylcholine(PC) silica column chromatography purification |
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