骨组织工程用PLGA多孔支架的制备及细胞毒性研究

制备能在骨组织工程研究中应用,并具有良好孔隙结构的块状聚（D,L-乳酸-CO-乙醇酸）（PLGA）多孔支架,探索出以冰粒子作为致孔剂,采用粒子滤出方法结合冷冻干燥工艺制备多孔支架的方法.首先将冰颗粒加入预冻的PLGA氯仿溶液中混合均匀,然后把混合物置于液氮中深度冷冻后冷冻干燥,制得多孔支架.对支架孔隙结构分析表明,该工艺制备的多孔支架无致孔剂残留、三维结构良好、孔径与孔隙可通过改变冰粒子的粒径和质量分数来控制;细胞毒性实验表明该多孔支架毒性在0～1级,可作为骨组织工程研究用多孔支架.

Fabrication and Cytotoxicity of Porous PLGA Scaffolds for Bone Tissue Engineering

To develop a massive poly(lactide-co-glycolide)(PLGA) copolymer scaffolds with good three-dimensional microstructure,so as to be used for bone tissue engineering.Using ice particulates as porogen material,porous scaffolds were fabricated by combination of porogen-leaching and freeze-drying.PLGA solutions in chloroform were mixed with ice particulates,then the mixture was freezed in liquid nitrogen,and porous scaffold was prepared by freeze-drying finally.The research of porous structure of the scaffolds showed that good three-dimensional microstructure and no porogen remains in the scaffold,pore size and porosity were determined by the size and mass fraction of ice particulates.The cytotoxicity of the scaffold was Class O or Class 1,So it can be used as a scaffold in the research of bone tissue engineering.