| WCX-HIC二维液相色谱柱对鸡蛋清中活性蛋白的分离纯化 |
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| 引用本文： | 王军,白泉. WCX-HIC二维液相色谱柱对鸡蛋清中活性蛋白的分离纯化[J]. 广东化工, 2012, 39(9): 152-153 |
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| 作者姓名： | 王军 白泉 |
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| 作者单位： | 1. 陕西省石油化工研究设计院质检中心,陕西西安710054 西北大学现在分离科学研究所,陕西西安710069
2. 西北大学现在分离科学研究所,陕西西安,710069 |
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| 基金项目： | 陕西省科技攻关2009K01-42，陕西省石油化工研究设计院青年创新基金QCX2011-010 |
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| 摘 要： | 使用一根色谱柱,采用离线单柱二维液相色谱技术对鸡蛋清中的3种蛋白质进行了的分离纯化。第一维采用离子交换色谱模式,可一步分离纯化得到抗生物素蛋白和溶菌酶,其纯度分别达到95.3%和95.6%。将在离子交换模式下不保留的组分再通过疏水色谱模式进行第二维分离,通过条件优化,一步可分离纯化得到球蛋白G2,其纯度可达到98.1%。
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| 关 键 词： | 液相色谱 二维色谱柱 鸡蛋清 |
Separation and Purification of Active Protein in the Egg by WCX-HIC 2D Colomn |
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| Wang Jun,Bai Quan. Separation and Purification of Active Protein in the Egg by WCX-HIC 2D Colomn[J]. Guangdong Chemical Industry, 2012, 39(9): 152-153 |
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| Authors: | Wang Jun Bai Quan |
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| Affiliation: | 1.Shaanxi Research Desing Institute of Petroleum and Chemical Industry,Xi’an 710054;2.Institute of Modern Separation Science,Northwest University,Xi’an 710069,China) |
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| Abstract: | Three proteins from egg white were separated and purified with 2D colomn offline,using one column initially.The egg white were separated in WCX mode firstly,and the lysozyme and advin can be purified with the purities of 95.3 % and 95.6 %,respectively.The fractions which were not retained in WCX mode on the 2D column were separated again in HIC mode with the 2D colume.Through conditional optimization,the protein G3 also can be purified successfully and the purity achieved to be 98.1 %. |
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| Keywords: | HPLC 2D colomn egg white |
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