Two-stage thermophilic bio-hydrogen and methane production from lime-pretreated oil palm trunk by simultaneous saccharification and fermentation |
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Authors: | Saruda Sitthikitpanya Alissara Reungsang Poonsuk Prasertsan |
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Affiliation: | 1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand;2. Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand;3. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Thailand |
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Abstract: | A simultaneous saccharification and fermentation (SSF) process was applied for thermophilic bio-hydrogen production from lime-pretreated oil palm trunk (OPT) by Thermoanaerobacterium thermosaccharolyticum KKU19. The SSF hydrogen fermentation conditions were optimized to maximize hydrogen yield (HY). Based on Plackett-Burman design, substrate loading and initial pH had significant effects on HY. The substrate loading and initial pH were further optimized using response surface methodology with a central composite design. The optimum conditions were a substrate loading, enzyme loading, inoculum concentration, initial pH and temperature of 4.6%, 10 filter paper unit (FPU)/g-OPT, 10% (v/v), 6.3 and 50 °C, respectively, which yielded the highest HY of 60.22 mL H2/g-OPT. Structural analysis showed that lime pretreatment and SSF decreased the crystallinity of OPT. Methane production was carried out following the hydrogen production to improve the energy yield from OPT. The results showed that methane production increased total energy yield from 0.65 to 11.79 kJ/g-OPT under the optimal conditions. |
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Keywords: | Lignocellulose Optimization Anaerobic digestion Biogas Energy analysis Thermophilic microorganisms |
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