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In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension |
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| Authors: | RE Wellinger JM Sogo |
| Affiliation: | Institut fur Zellbiologie, ETH-H?nggerberg, CH-8093 Zurich, Switzerland. |
| Abstract: | By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp. |
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