Fast, ultrasensitive virus detection using a Young interferometer sensor |
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Authors: | Ymeti Aurel Greve Jan Lambeck Paul V Wink Thijs van Hövell Stephan W F M Beumer Tom A M Wijn Robert R Heideman Rene G Subramaniam Vinod Kanger Johannes S |
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Affiliation: | Biophysical Engineering, MESA+ Institute for Nanotechnology and Institute for Biomedical Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands. A.Ymeti@utwente.nl |
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Abstract: | We report the application of an integrated optical Young interferometer sensor for ultrasensitive, real-time, direct detection of viruses. We have validated the sensor by detecting herpes simplex virus type 1 (HSV-1), but the principle is generally applicable. Detection of HSV-1 virus particles was performed by applying the virus sample onto a sensor surface coated with a specific antibody against HSV-1. The performance of the sensor was tested by monitoring virus samples at clinically relevant concentrations. We show that the Young interferometer sensor can specifically and sensitively detect HSV-1 at very low concentrations (850 particles/mL). We have further demonstrated that the sensor can specifically detect HSV-1 suspended in serum. Extrapolation of the results indicates that the sensitivity of the sensor approaches the detection of a single virus particle binding, yielding a sensor of unprecedented sensitivity with wide applications for viral diagnostics. |
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