| 微生物发酵谷氨酰胺转胺酶的超滤过程研究 |
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| 引用本文： | 荣绍丰,常忠义,高红亮,柏俊华,瞿伟菁.微生物发酵谷氨酰胺转胺酶的超滤过程研究[J].食品与发酵工业,2007,33(6):9-12. |
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| 作者姓名： | 荣绍丰 常忠义 高红亮 柏俊华 瞿伟菁 |
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| 作者单位： | 1. 上海应用技术学院生物与食品工程系,上海,200235
2. 华东师范大学生命科学学院,上海,200062 |
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| 摘 要： | 将Streptoverticillium mobaraense发酵得到的谷氨酰胺转胺酶粗酶液进行凝胶层析,采用SDS-PAGE电泳得到了谷氨酰胺转胺酶的分子质量在37ku左右,pI值在8.7～9.0。对超滤过程TGase的酶活损失和超滤膜通量综合考虑,确定超滤过程温度控制在8℃左右比较适宜,在此基础上,考察了对膜通量有较大影响的酶液pH值和超滤压力等因素。实验结果表明,将料液pH调节到7.5左右,压力控制在0.20～0.25MPa比较理想。
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| 关 键 词： | 谷氨酰胺转胺酶 超滤 酶活 |
| 修稿时间： | 2006-11-08 |
Studies on Application of Utrafiltration in Transglutaminase Fermented by Microorganism |
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| Rong Shaofeng,Chang Zhongyi,Gao Hongliang,Bai Junhua,Zai Weiqing.Studies on Application of Utrafiltration in Transglutaminase Fermented by Microorganism[J].Food and Fermentation Industries,2007,33(6):9-12. |
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| Authors: | Rong Shaofeng Chang Zhongyi Gao Hongliang Bai Junhua Zai Weiqing |
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| Affiliation: | 1.Depaitment of Biotechnology and Food Technolog,Shanghai Institute of Technology,Shanghai 200235,China;2.School of Life Science,East China Normal University,Shanghai 200062,China |
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| Abstract: | The crude transglutaminase broth fermented by Streptoverticillium mobaraense was separated and purified by gel chromatography.The SDS-PAGE results showed that the molecular weight of transglu- taminase and the value of pI were 37ku and 8.7～9.0 respectively.The main parameters of the ultra-filtration including temperature,pH value and pressure on the loss of the transglutaminase activity and membrane per- meating flux were analyzed and the results are(8±2)℃,7.5 and 0.20～0.25MPa respectively. |
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| Keywords: | transglutaminase utralfiltration enzyme activity |
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