影响纤细角毛藻生长的因素及其脂肪酸组成的研究

对影响纤细角毛藻生长的温度、pH、通气率、光强等培养条件进行了研究,获得了纤细角毛藻培养的优化条件为温度23℃,光照强度8mW/cm2,通气速率06vvm;考察了培养条件对纤细角毛藻脂肪酸的组成含量的影响,结果显示光照强度、通气量及培养时间对二十碳五烯酸和花生四烯酸的含量有一定的影响,纤细角毛藻中EPA和AA的总量可占藻体干重的667%,高通气量及高的光照强度有利于花生四烯酸的积累,而低光照有利于二十碳五烯酸的积累。

Research on Growth Factor and Fatty Acids of Chaetoceros gracilis

The effect of temperature, pH and light density on the growth of Chaetoceros gracilis was investigated, and the appropriate culture conditions were determined as temperature 23℃,light density 8 mW/cm2 and aeration rate 0.6?vvm. The effect of culture conditions on fatty acids composition and content of Chaetoceros gracilis were also studied. The results showed that light density, aeration rate and culture period all had certain effect on the content of EPA and AA. The content accounting for cell dry weight can reach 6.67%.High aeration rate and high light density were availed of accumulation of AA, but low light density was availed of accumulation of EPA.