Modelling and quality assessment of spots in digital images — With application to DNA microarrays |
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| Authors: |
ystein Gjerstad got Aakra Ulf Indahl |
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| Affiliation: | aDepartment of Chemistry, Biotechnology and Food Sciences, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway;bDepartment of Mathematical Sciences and Technology, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway;cCentre for Biospectroscopy and Data Modelling Oslovn. 1, N-1430 Ås, Norway |
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| Abstract: | Based on a recently developed spot segmentation method, we here present a new approach to modelling of individual spots in digital images, e.g. images of DNA microarrays. From the model parameter estimates and residuals we have developed an expedient approach to automatic quality assessment and identification of corrupted spots. The suggested approach to quality control is shown to give a statistically significant decrease of the variance in gene expression log-ratio estimates for three different DNA microarray datasets. |
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| Keywords: | Spot modelling DNA microarray Quality control |
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