Fabricating a new immobilization matrix based on a conjugated polymer and application as a glucose biosensor

A new monomer named 4-(dihexylamino)9,12-di(thiophen-2-yl)-7H-benzo[de]benzo[4,5]imidazo[2,1-a]isoquinolin-7-one (ThBN) is synthesized and used as a potential glucose biosensor after electropolymerization of the ThBN on the graphite pencil electrode. The amount of glucose is determined according to the decrease in the amount of oxygen by using cyclic voltammetry technique. Herein, conjugated polymer of ThBN is used as a immobilization matrix. The synthesized PThBN is found to be effective enzymatic biosensor having wide linear glucose determination range between 2.975 × 10⁻³ and 2.087 mM with a limit of detection of 0.0304 mM and a sensitivity of 0.1326 μA/mM cm². This potential enzymatic biosensor has been also tested in commercial samples and found to be useful to detect the glucose concentration.