Purification of Soybean Phosphatidylcholine Using D113-III Ion Exchange Macroporous Resin Packed Column Chromatography |
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| Authors: | Yuanfa Liu Liang Shan Xingguo Wang |
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| Affiliation: | (1) State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu Province, People’s Republic of China |
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| Abstract: | Ten ion-exchange resins were compared for their abilities to separate soybean phosphatidylcholine (PC). D113-III resin was
selected for PC purification among ten resins tested. The optimum PC adsorption conditions were: concentration of PC solution,
3 mg/mL; pH of PC solution, 7.5; and adsorption flow rate, 1 mL/min. The optimum PC desorption conditions were: eluent, 95%
ethanol aqueous solution; and elution flow rate, 1 mL/min. Under these conditions, the PC content in the product and the amount
of product were 94 and 80% (wt.%), respectively, and the D113-III resin was able to be used at least six times for purifying
PC. The adsorption and desorption characteristics were also studied. |
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| Keywords: | Ion-exchange resin Phosphatidylcholine Phospholipid separation |
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