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Automated single nucleotide polymorphism typing using bead array in capillary tube |
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| Authors: | Yasuo Kagawa Mami Hiraoka Yukiko Miyashita-Hatano Madoka Shishido-Oki Mamiko Yoshida Susumu Kondou Mika Sugiura Kazumi Sawakami-Kobayashi Masaaki Takahashi Hideji Tajima Masafumi Yohda |
| Affiliation: | 1 High Technology Research Center of Kagawa Nutrition University, 3-9-21 Chiyoda, Sakado, Saitama 350-0288, Japan;2 Precision Systems Science Co., Ltd., 88 Kamihongou, Matsudo, Chiba 271-0064, Japan;3 Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-6 Naka-cho, Koganei, Tokyo 184-8588, Japan |
| Abstract: | A low-cost and simple on-site technique for genotyping single nucleotide polymorphisms (SNPs) was developed. The technique is based on allele-specific primer PCR and the recently developed bead arrays in a single tip technique. The performance of the method was verified by genotyping four SNPs that correlate with cardiovascular diseases. |
| Keywords: | SNP Allele-specific primer Genotyping Beads array Automation |
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