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Combination of cytomegalovirus enhancer with human cellular promoters for gene-induced chondrogenesis of human bone marrow mesenchymal stem cells |
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| Authors: | Ryosuke Iwai Yoko Kumagai Masashi Fujiwara Shigeyuki Wakitani Mutsumi Takagi |
| Affiliation: | 1 Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan;2 Orthopedic Surgery, Osaka City University School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka, 545-8585, Japan |
| Abstract: | High-expression plasmid vectors for human mesenchymal stem cells (MSCs) were constructed by combination of cytomegalovirus immediate-early enhancer with cellular promoters. MSCs transfected with the vector showed higher transgene production of a cytokine, which increased the differentiation level to chondrocytes. |
| Keywords: | Cartilage regeneration Gene therapy Human mesenchymal stem cell Nonviral-transfection CMV enhancer Human cellular promoter |
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