The inclusion of fetal bovine serum in gelatin/PCL electrospun scaffolds reduces short‐term osmotic stress in HEK 293 cells caused by scaffold components |
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| Authors: | Young Hun Kim Do‐Hyung Kim Junmo Hwang Hyeng‐Soo Kim Ga Young Lim Zae Young Ryoo Sang‐Un Choi Sanggyu Lee |
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| Affiliation: | 1. School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea;2. School of Physics and Energy Sciences, Kyungpook National University, Daegu, Republic of Korea;3. Korea Research Institute of Chemical Technology, Daejeon 305‐600, Republic of Korea |
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| Abstract: | Components of gelatin/polycaprolactone (PCL) electrospun scaffolds are released to surrounding media and cause osmotic changes that adversely affect cell viability and proliferation. In this study, the physiological properties of gelatin/PCL scaffolds were investigated by qRT‐PCR and by performing cellular studies on HEK 293 cells. Components released from gelatin/PCL scaffolds were found to induce osmotic stress response in these cells. However, osmotic stress was inhibited by adding fetal bovine serum (FBS) to scaffolds. In addition, focal adhesion related genes were found to be up‐regulated in HEK 293 cells on gelatin/PCL/20% FBS scaffolds, and this induced the down‐regulations of cell‐death related genes. Furthermore, the inclusion of 20% FBS improved the viabilities of HEK 293 cells on gelatin/PCL scaffolds. This study indicates that adding FBS to gelatin/PCL scaffolds improves scaffold bio‐affinity. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 |
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| Keywords: | biocompatibility biodegradable properties and characterization electrospinning biomaterials |
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