Synthesis and properties of an insoluble chitosan resin modified by azamacrocycle copper(II) complex for protein hydrolysis |
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| Authors: | Xingyu Wang Dongfeng Wang Peng Liang Xingguo Liang |
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| Affiliation: | 1. Department of Food Chemistry and Nutrition, College of Food Science and Technology, Ocean University of China, Qingdao 266003, People's Republic of China;2. Department of Food Molecular Biology, College of Food Science and Technology, Ocean University of China, Qingdao 266003, People's Republic of China |
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| Abstract: | We reported the synthesis process and the proteolytic ability of a novel crosslinked chitosan resin modified by azamacrocycle copper complex (CMCR). Characterization of CMCR showed that the Cu(II)Cyclen complex had successfully bound to the resin matrix covalently. Its proteolytic activity toward peptide bonds was evaluated by investigating the hydrolysis of bovine serum albumin in heterogeneous media. The maximum‐observed pseudo‐first‐order rate constant (kobs) was achieved under 60°C, pH 6.0, where protein was hydrolyzed into smaller peptides and amino acids confirmed by SDS–PAGE and ninhydrin reaction. The results indicated that the hydrolyzing efficiency delivered by CMCR was 4 × 105 times higher than that of spontaneous hydrolysis. CMCR also featured a desirable stability for chelating Cu2+ ions, which paved the way for its feasible recovery process and good reusability. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci, 2013 |
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| Keywords: | proteins resins crosslinking polysaccharides |
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