Synthesis,characterization, and antifungal activity of nystatin—gum arabic conjugates |
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| Authors: | Jovana Stefanovic Dragica Jakovljevic Gordana Gojgic‐Cvijovic Miodrag Lazic Miroslav Vrvic |
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| Affiliation: | 1. IChTM, Department of Chemistry, University of Belgrade, Njegoseva 12, P. O. Box 473, 11000 Belgrade, Serbia;2. Faculty of Technology, Department of Food Technology and Biotechnology, University of Nis, Bulevar oslobodjenja 124, 16000 Leskovac, Serbia;3. Faculty of Chemistry, Department of Biochemistry, University of Belgrade, Studentski trg 12–16, P. O. Box 51, 11158 Belgrade, Serbia |
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| Abstract: | Nystatin, a polyene tetraene antibiotic widely used in the treatment of mycoses, was coupled with oxidized polysaccharide gum Arabic, by forming Schiff base structures between amine groups of antibiotics and aldehyde groups of modified carbohydrate. Imine conjugates synthesized in this way were reduced with sodium borohydride to secondary amines. Two imine and two amine conjugates were obtained with different nystatin content. The conjugates were characterized by UV–Vis, FTIR, 1H NMR spectroscopy, and thermogravimetric analysis. Solubility in water, unlike nystatin, and significant activity against Candida albicans and Aspergillus niger with minimum inhibitory concentrations in range of 3.125–6.25 μg mL?1 and 6.25–25 μg mL?1, respectively, indicate that the chemical integrity and the biological function of these compounds were retained. A comparison of stability of the conjugates in the dry form, solution and under different pH values showed that the conjugates exhibited better stability than pure drug. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 |
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| Keywords: | nystatin polysaccharide coupling solubility antifungal activity |
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