AFLATOXIN LIKE FLUORESCENT SUBSTANCES IN SPICES |
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| Authors: | M S MADHYASTHA RAMESH V BHAT |
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| Affiliation: | Food and Drug Toxicology Research Centre, National Institute of Nutrition Indian Council of Medical Research, Hyderabad-500007, India |
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| Abstract: | To minimize the risk of misidentifying some blue fluorescent substances inherent in spices as aflatoxin during analysis, several confirmatory chemical tests and developing solvent systems were evaluated. Chemical confirmation with 20% HCl, 20% H2SO4 and TFA and TLC development in chloroform + acetone (95:5 v/v) followed by anhydrous diethyl ether (multiple development) were useful to obviate the interfering fluorescent substances. |
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