Observations of casein micelles in skim milk concentrate by transmission electron microscopy |
| |
Authors: | AO Karlsson R Ipsen Y Ardö |
| |
Affiliation: | Dairy Technology, Department of Food Science, Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 305, 1958 Fredriksberg C, Denmark |
| |
Abstract: | The microstructure of casein micelles in ultrafiltrated (UF) skim milk concentrate at pH 6.5 and 5.8 was investigated by transmission electron microscopy using three different preparation methods. The volume fraction of the casein micelles in the UF concentrate was 62.8% (v/v) at pH 6.5 and fixation by glutaraldehyde revealed the close packing of micelles in the UF concentrate as well as a higher degree of micelle aggregation at pH 5.8. No details of the microstructure of the micellar surface or core could, however, be observed. Freeze-fracture of cryoprotected, i.e. glycerol, UF concentrate on the other hand, exposed the finer structures of the micellar core but no pH dependent differences were observed. As cryoprotection includes a dilution of the sample with glycerol, the packing of the micelles in the UF concentrate could not be observed. Undiluted UF concentrate exposed to rapid freezing using a propane jet followed by freeze-fracture exhibited development of ice crystals but rough areas on the micrographs were identified as fractured casein micelles. The micellar core appeared rougher and differences in the micellar core microstructure due to changing pH could be observed when this preparation method was used. |
| |
Keywords: | Casein micelles Ultrafiltration Microstructure Transmission electron microscopy |
本文献已被 ScienceDirect 等数据库收录! |
|