Free radical scavenging capacity and antioxidant enzyme activity in deerberry (Vaccinium stamineum L.) |
| |
Authors: | Shiow Y Wang James R Ballington |
| |
Affiliation: | a Fruit Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705-2350, USA b Horticultural Science Department, North Carolina State University, Raleigh, NC 27695-7609, USA |
| |
Abstract: | Fruit from three genotypes (‘B-76’, ‘B-59’ and ‘SHF-3A’) of deerberry Vaccinium stamineum L.] were evaluated for fruit quality, total anthocyanin and phenolic contents, antioxidants, antioxidant capacity, and antioxidant enzyme activity. The fruit soluble solids, titratable acids, total anthocyanins, and total phenolic contents varied with genotypes. Cyanidin 3-galactoside and cyanidin 3-arabinoside were the two predominant anthocyanins. Resveratrol was also found in deerberries. Among the three genotypes, ‘B-76’ had higher amount of anthocyanins, phenolic compounds and resveratrol than ‘B-59’ and ‘SHF-3A’. Deerberries contained potent free radical scavenging activities for 2,2-Di (4-tert-octylphenyl)-1-picrylhydrazyl (DPPH ), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS +), peroxyl radical (ROO ), superoxide radicals ( ), hydrogen peroxide (H2O2), hydroxyl radicals ( OH), and singlet oxygen (1O2) radicals and also had high activities of antioxidant enzymes including glutathione-peroxidase (GSH-POD), glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (AsA-POD), guaiacol peroxidase (G-POD), monodehydroascorbate radical reductase (MDAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR)] and non-enzyme antioxidants ascorbic acid (ASA) and reduced glutathione (GSH)]. Antioxidant capacities were highly correlated to antioxidant enzymes activities. Among the three genotypes, ‘B-76’ had the highest level of antioxidants and antioxidant enzyme activity. |
| |
Keywords: | Vaccinium stamineum Sugar Acid Anthocyanin Phenolics Radical scavenging Antioxidant enzyme |
本文献已被 ScienceDirect 等数据库收录! |
|