| 一种新型固定化青霉素酰化酶的性能及动力学常数的测定 |
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| 引用本文： | 曲红波,丛威,王贞,欧阳藩.一种新型固定化青霉素酰化酶的性能及动力学常数的测定[J].过程工程学报,1999,20(1):51-56. |
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| 作者姓名： | 曲红波 丛威 王贞 欧阳藩 |
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| 作者单位： | 中科院化工冶金研究所生化工程国家重点实验室,北京,100080 |
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| 摘 要： | 测定了一种以自制的新型载体制备的固定化青霉素酰化酶的基本性质和各项动力学参数．固定化酶的稳定性与中科院微生物研究所用同种游离酶制备的纤维状固定化酶相当；其活性对温度和ｐＨ值的变化不如游离酶敏感；米氏常数、底物抑制常数、苯乙酸和６－氨基青霉烷酸的抑制常数分别为：０．６７９，７４９，２．２４和９２ｍｍｏｌ／Ｌ．
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| 关 键 词： | 固定化酶 青霉素酰化酶 载体 6-氨基青霉烷酸 |
DETERMINATION OF PROPERTIES AND KINETIC CONSTANTS OF IMMOBILIZED PENICILLIN AMIDASE ON NOVEL MICROBEAD CARRIER |
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| QU Hongbo,CONG Wei,WANG Zhen,OUYANG Fan.DETERMINATION OF PROPERTIES AND KINETIC CONSTANTS OF IMMOBILIZED PENICILLIN AMIDASE ON NOVEL MICROBEAD CARRIER[J].Chinese Journal of Process Engineering,1999,20(1):51-56. |
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| Authors: | QU Hongbo CONG Wei WANG Zhen OUYANG Fan |
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| Abstract: | The properties of WTBX]
Bacillus megarium penicillin amidase which is immobilized on a novel microbead carrier
invented in the authors'laboratory were studied. The kinetic parameters were determined.
The stability of the immobilized enzyme is the same as that of fibrous IME made from same
free enzyme. The activity of the IME is not as susceptible to the changes of temperature
or pH as that of free enzyme. The Michaelis Menton constant and the inhibition constants
of the substrate, phenylacetic acid (PAA) and6-APA are 0.679, 749, 2.24 and 92 mmol/L,
respectively. |
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| Keywords: | Immobilized enzyme Penicillin amidase Carrier 6-APA |
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