| PCR 法检测食品中大肠杆菌O157:H7 |
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| 引用本文： | 巢强国,杨学明,葛宇,熊薇,曲勤凤,王瑞元. PCR 法检测食品中大肠杆菌O157:H7[J]. 食品科学, 2010, 31(8): 212-215. DOI: 10.7506/spkx1002-6300-201008047 |
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| 作者姓名： | 巢强国 杨学明 葛宇 熊薇 曲勤凤 王瑞元 |
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| 作者单位： | 1.上海市质量监督检验技术研究院 2.华东理工大学生物工程学院 |
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| 基金项目： | 上海市质量监督检验局项目(2007-21) |
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| 摘 要： | 对大肠杆菌O157:H7 型菌株的各毒力基因及基因组中的特异序列进行了设计和比对，确定292bp 的检测引物。常规定性PCR 和实时定量PCR 证明该引物特异性强。模拟样品的前增菌实验结果表明本方法可以在原样品活菌浓度约2.0CFU/mL 时通过16h 增菌后检测出来，总的检测时间可以控制在24h 内。本实验建立的PCR 方法可用于食品中大肠杆菌O157:H7 的快速测定。
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| 关 键 词： | 大肠杆菌O157:H7 食源性致病菌 特异序列 PCR |
| 收稿时间： | 2009-03-03 |
Rapid PCR Detection of Enterohemorrhagic Escherichia coli O157:H7 in Foods |
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| CHAO Qiang-guo,YANG Xue-ming,GE Yu,XIONG Wei,QU Qin-feng,WANG Rui-yuan. Rapid PCR Detection of Enterohemorrhagic Escherichia coli O157:H7 in Foods[J]. Food Science, 2010, 31(8): 212-215. DOI: 10.7506/spkx1002-6300-201008047 |
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| Authors: | CHAO Qiang-guo YANG Xue-ming GE Yu XIONG Wei QU Qin-feng WANG Rui-yuan |
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| Affiliation: | 1. Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China；2. School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | Genomic comparisons were carried out on Escherichia coli virulence genes and genome-specific sequences,which were located in the genomes of Escherichia coli serotype O157:H7 strains released in the GenBank.One pair of primers specific for ECs3032 genes was exploited for the amplification to give a 292 bp PCR product.Conventional qualitative PCR and real-time quantitative PCR assays showed higher specificity of the primers.The assays were used for detecting Escherichia coli O157:H7 in ground beef initially i... |
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| Keywords: | enterohemorrhagic Escherichia coli O157:H7 foodborne pathogenic bacteria specific sequence PCR |
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