聚乙二醇改性聚乳酸幽门螺杆菌微球疫苗的制备与体外控释

目的 应用聚乳酸与聚乙二醇共聚物（ＰＥＬＡ）包裹Ｈｐ超声上清液，制备 Ｈｐ口服微球疫苗，探讨共 聚物ＰＥＬＡ总相对分子质量ｒ、ＰＥＧ含量与不同相对分子质量ＰＥＬＡ共聚物的相对分子质量分布（Ｍｒ／Ｍｎ）对微球疫 苗体外释药特性的影响。方法　采用复乳法溶剂挥发技术，制备Ｈｐ微球疫苗，用电子显微镜与扫描电镜观测微球 疫苗的粒径，用紫外光谱分光光度法测定抗原包裹效率与Ｈｐ抗原释放量，并在ｐＨ７．３、浓度为 ０．０１ｍｏｌ／Ｌ的ＰＢＳ溶 液中作体外控释实验。结果 Ｈｐ微球疫苗抗原包裹效率在７５％左右，平均粒径在５１μｍ以下。若ＰＥＧ量低于 １０％，ＰＥＬＡ组成对微球疫苗的粒径和抗原包裹效率影响不大。结论 Ｈｐ抗原体外释放量与 ＰＥＬＡ中ＰＥＧ的量和 相对分子质量、ＰＥＬＡ的相对分子质量分布成正比，而与ＰＥＬＡ的总相对分子质量成反比。

Preparation and Controlled Release In Vitro of Hp-Loaded Poly(Kactude)-Poly ( Ethylene Glycol ) -Poly ( Lactide ) Microspheres

Objective To prepare Hp-loaded PLA and PELA microsphers and evaluate the controlled release in vitro of it.Methods Hp-loaded PLA and PELA microsphers were prepared by encapsulating Hp ly-sates by a double emulsion/solvent evaporation method, and the sizes of diem were observed by electron micros-copy and scanning electron microscopy.The encapsulation efficacy and release of Hp antigen were determined by ultraviolet spectroscopy, and the controlled release test of it was performed in 0.01 mol/L PBS ( pH7. 3). Results The encapsulation efficacy of Hp antigen was about 75% , and the average size of microsphers was less than 51 μm. If PEG content was less than 10% ,the composition of PELA showed no significant influence on the size of microsphers and encapsulation efficacy of Hp antigen. Conclusion The amount of Hp antigen released in vitro is positively related to the content and molecular weight of PEG in PELA as well as the molec-ular weight distribution of PELA. However, it is negatively related to the total molecular weight of PELA.