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Bacterial hydrogen production in recombinant Escherichia coli harboring a HupSL hydrogenase isolated from Rhodobacter sphaeroides under anaerobic dark culture |
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| Authors: | Soo Youn Lee Hyun Jeong Lee Jae-Min Park Jin Hyung Lee Jin-Soo Park Hwa Sung Shin Yang-Hoon Kim Jiho Min |
| Affiliation: | 1. Department of Bioprocess Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561 756, South Korea;2. Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561 756, South Korea;3. Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361 763, South Korea;4. Bio-IT convergence center, Korea Institute of Ceramic Engineering and Technology (KICET), 233-5 Gasan-Dong, Geumcheon-Gu, Seoul 153 801, South Korea;5. Department of Environmental Engineering, College of Engineering, Sangmyung University, 300 Anseo-dong, Dongnam gu, Cheonan, Chungnam Province, 330 720, South Korea;6. Department of Biological Engineering, Inha University, Incheon 402 751, South Korea |
| Abstract: | In this study, recombinant plasmid was constructed to analyze the effect of hydrogen production on the expression HupSL hydrogenase isolated from Rhodobacter sphaeroides in Escherichia coli. Although most of recombinant HupSL hydrogenase was produced as inclusion bodies the solubility of the protein increased significantly when the expression temperature shifted from 37 °C to 30 °C. Hydrogen production by expression of HupSL hydrogenase from recombinant E. coli increased 20.9-fold compared to control E. coli and 218-fold compared to wild type R. sphaeroides under anaerobic dark condition. The results demonstrate that HupSL hydrogenase, consisting of small and large subunits of hydrogenase isolated from R. sphaeroides, increases hydrogen production in recombinant E. coli. In addition conditions for enhancing the activity of HupSL hydrogenase in E. coli were suggested and were used to increase bacterial hydrogen production. |
| Keywords: | Hydrogen HupSL hydrogeanse Rhodobacter sphaeroides Recombinant E. coli |
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