Fermentative hydrogen production by diverse microflora |
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Authors: | Bita Baghchehsaraee George NakhlaDimitre Karamanev Argyrios Margaritis |
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Affiliation: | Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario N6A 5B9, Canada |
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Abstract: | In this study, hydrogen production with activated sludge, a diverse bacterial source has been investigated and compared to microflora from anaerobic digester sludge, which is less diverse. Batch experiments were conducted at mesophilic (37 °C) and thermophilic (55 °C) temperatures. The hydrogen production yields with activated sludge at 37 °C and 55 °C were 0.56 and 1.32 mol H2/mol glucose consumed, respectively. While with anaerobically digested sludge hydrogen yield was 2.18 mol H2/mol glucose consumed at 37 °C and 1.25 mol H2/mol glucose consumed at 55 °C. The results of repeated batch experiments for 615 h resulted in average yields of 1.21 ± 0.62 and 1.40 ± 0.16 mol H2/mol glucose consumed for activated sludge and anaerobic sludge, respectively. The hydrogen production with activated sludge was not stable during the repeated batches and the fluctuation in hydrogen production was attributed to formation of lactic acid as the predominant metabolite in some batches. The presence of lactic acid bacteria in microflora was confirmed by PCR-DGGE. |
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Keywords: | Biological hydrogen production Repeated batch Activated sludge Anaerobically digested sludge PCR-DGGE |
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