白鱼腐败细菌的分离与鉴定

通过对不同温度条件下白鱼（Anabarilius）菌落数量的统计发现， 与37 ℃相比，25 ℃培养条件更有利于白鱼携带细菌的生长，也更容易导致白鱼的腐败。通过对分离自腐败的白鱼中21 株细菌的16S rDNA序列分析后，最终确定得到了11 株不同的菌株。同时结合生化鉴定结果，将这些菌种分属7 个不同的属：2 株摩根氏菌（Morganella），3 株变形杆菌（Proteus），2 株漫游球菌（Vagococcus），1 株葡萄球菌（Staphylococcu），1 株泰瑞芽孢杆菌（Terribacillus），1 株赖氨酸芽孢杆菌（Lysinibacillus），1 株透明颤菌（Vitreoscilla）。其中摩根氏菌和变形杆菌同属肠杆菌科，是腐败白鱼的优势菌，可能是白鱼的特定腐败菌 （specific spoilage organisms，SSO）。

Isolation and Identification of Spoilage Organisms in Whitefish

The growth of spoilage organisms is the main factor causing the spoilage of aquatic products. Colony counting
showed that the growth rate of spoilage microorganisms in whitefish was much faster at 25 ℃ than at 37 ℃, and therefore
fish spoilage occurred more easily at the lower temperature. Through 16S rDNA sequence analysis, 21 strains isolated from
putrid whitefish were identified as 11 different strains belonging to seven different genera: two strains of Morganella sp.,
three strains of Proteus sp., two strains of Vagococcus sp., one strain of Staphylococcu sp., one strain of Terribacillus sp., one
strain of Lysinibacillus sp., one strain of Vitreoscilla sp.. In this study, the strains of Morganella sp. and Proteus sp. were the
dominant spoilage bacteria and may be the SSO (specific spoilage organisms) in whitefish.