蓝莓花青素氯化锦葵色素在人脐静脉内皮细胞中的抗氧化作用

目的：研究氯化锦葵色素对人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVEC）中活性氧（reactive oxygen species，ROS）和黄嘌呤氧化酶-1（xanthine oxidase-1，XO-1）含量的影响，探讨氯化锦葵色素在血管内皮细胞中的抗氧化作用。方法：体外培养HUVEC，分别设定空白组（仅加入二甲基亚砜），血管紧张素Ⅱ（angiotensinⅡ，AngⅡ）刺激组（10－2 μmol/L），终浓度为1、5、10 μmol/L氯化锦葵色素组，以及终浓度为1、5、10 μmol/L氯化锦葵色素联合AngⅡ刺激（10－2 μmol/L）组。采用荧光免疫法测定HUVEC内ROS含量的变化，酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）测定细胞上清液中可溶性XO-1含量的变化，Western blotting法检测细胞中XO-1含量的变化。结果：氯化锦葵色素处理HUVEC时，细胞ROS和XO-1含量降低；AngⅡ引起HUVEC内ROS和上清液中XO-1含量显著增加，各浓度氯化锦葵色素预处理能有效抑制ROS和XO-1含量的增加。结论：氯化锦葵色素在HUVEC中具有抑制ROS和XO-1的作用，从而达到抗氧化、保护内皮细胞的功效。蓝莓花青素氯化锦葵色素具有抑制ROS和XO-1的作用，为蓝莓的开发利用提供了理论依据。

Antioxidant Effect of Blueberry Malvidin Chloride in Human Umbilical Vein Endothelial Cells

Objective: The effect of malvidin chloride (Mv-Cl) extracted from blueberries on the amounts of reactive oxygen
species (ROS) and xanthine oxidase-1 (XO-1) in human umbilical vein endothelial cells (HUVECs) was investigated to
explore the antioxidant potential of the anthocyanin compound. Methods: HUVECs were in vitro cultured in the presence
of Mv-Cl (1, 5 and 10 μmol/L), angiotensinⅡ(Ang II, 10-2 μmol/L) and their combination, respectively. DMSO was used
as control. The ROS content in the cells was detected by immunofluorescence. The soluble XO-1 protein content in the cell
culture supernatant was detected by ELISA. The protein expression of XO-1 in the cells was assessed by Western blotting.
Results: The content of ROS and the protein expression of XO-1 in HUVECs were decreased when treated with low
concentration of Mv-Cl. Ang II significantly increased ROS and XO-1 content, and Mv-Cl appeared to specifically down-regulate
the production of ROS and XO-1. Conclusion: Malvidin chloride can prevent endothelial dysfunction by inhibiting ROS and XO-1.
Therefore, blueberry anthocyanin may be a new inhibitor of ROS and XO-1, which provides a theoretical basis for the application
of blueberry anthocyanins to prevent cardiovascular diseases as a functional food or nutraceutical ingredient.