不同培养条件对禾谷镰刀菌产玉米赤霉烯酮的影响

研究不同培养条件对禾谷镰刀菌（Fusarium graminearum）产玉米赤霉烯酮（zearalenone，ZEN）能力的影响。选取不同的培养基配比、培养时间、培养温度、摇床转速及光暗反应对禾谷镰刀菌进行培养。在单因素试验的基础上，分别采用正交试验设计和响应面设计的方法进行统计学分析。结果表明：禾谷镰刀菌的最适培养基为每升超纯水含葡萄糖60 g、KNO3 1.5 g、酵母浸出膏1.0 g、蛋白胨20 g、NaNO3 6.0 g、MgSO4 0.5 g、K2HPO4·3H2O 1.0 g、KCl 0.5 g、Fe2(SO4)3 0.025 g；当摇床转速为92 r/min、照明时间为10 h/d、培养温度为22.9 ℃时，20 d毒素质量浓度可达到249.80 μg/L。

Effect of Different Culture Conditions on the Production of Zearalenone by Fusarium graminearum

In the present study, the effects of different incubation conditions on the production of zearalenone (ZEN) by
Fusarium graminearum were explored. Culture medium, incubation time, incubation temperature, shaking speed and
light irradiation time were selected as important parameters affecting ZEN production. Single factor method was used in
combination with orthogonal array design and response surface methodology to optimize the medium composition and
culture conditions employing statistical analysis, respectively. The results showed that the optimal culture medium for
Fusarium graminearum was comprised of 60 g/L C6H12O6, 1.5 g/L KNO?, 1.0 g/L yeast extract paste, 20 g/L peptone,
6.0 g/L NaNO3, 0.5 g/L MgSO4, 1.0 g/L K2HPO4·3H2O, 0.5 g/L KCl, and 0.025 g/L Fe2(SO4)3 in 1.0 L of ultrapure water.
The concentration of ZEN was 249.80 μg/L when the cultivation was carried out for 20 days with a shaking speed of
92 r/min, 22.9 ℃ under light irradiation for 10 h a day.