石榴叶多酚对小鼠急性酒精性肝损伤的保护作用

目的：探究石榴叶多酚对酒精所致小鼠急性肝损伤的保护作用。方法：将60 只小鼠随机分为空白组，模型组，石榴叶多酚2低、中、高剂量组（100、200、400 mg/（kg•d））和阳性对照组（联苯双酯滴丸，150 mg/（kg•d）），每组10 只。空白组与模型组小鼠灌胃去离子水（0.1 mL/（10 g•d）），连续灌胃30 d；第31天，除空白组外，其余组小鼠均灌胃50%乙醇（12 mL/kg）建立急性肝损伤模型，12 h后，取小鼠眼球血清测定生化指标，取肝脏观察肝组织的超微结构。结果：模型组小鼠肝脏天冬氨酸氨基转移酶（aspartate aminotransferase，AST）、丙氨酸氨基转移酶（alanine aminotransferase，ALT）活力及血清中甘油三酯（triglyceride，TG）水平、ALT和AST活力明显升高，亚显微观察显示小鼠肝细胞膜结构异常，线粒体畸形，内质网明显扩张，内质网膜上附着的核糖体脱落，肝细胞内出现较多脂滴；随着给药剂量的增加，石榴叶多酚2各剂量组对上述情况的改善作用也不断加强。结论：石榴叶多酚2对小鼠急性酒精性肝损伤具有一定的保护作用。

Protective Effect of Polyphenols from Punica granatum L.Leaves against Alcohol-Induced Acute Hepatic Injury in Mice

Objective: To investigate the protective effect of purified polyphenols from Punica granatum L. leaves 2 (PPPL2)
on acute alcoholic hepatic injury in mice. Methods: Totally 60 mice were randomly divided into control group, model group,
low-, middle- and high-dose PPPL2 groups, and positive control group (bifendate, 150 mg/(kg·d)) of 10 mice each. The
mice from the control and model groups were administrated with deionized water (0.1 mL/(10 g·d)) daily by gavage for
30 consecutive days. The mice from three different dose groups were administrated with PPPL2 at 100, 200 and 400 mg/(kg·d)),
respectively. The mice from the positive control group were administrated with bifendate (150 mg/(kg·d)). The model of acute
alcoholic liver injury was established on the 31st day, and all mice were gavaged with 50% alcohol (12 mL/kg), except for the
control group. Biochemical indicators of ocular blood in mice were observed. Results: Compared with the model group, the levels
of glutamic-oxaloacetic transaminase (AST), alanine transaminase (ALT), and triglyceride (TG) were obviously decreased in the
treatment groups, and the mice from these treatment groups had reduced expansion of mitochondria and glycogen deposition. In
addition, the endoplasmic reticulum was not obviously outspread and lipid droplets were reduced in hepatic cells. Conclusion:
Polyphenols from pomegranate leaves have protective effect on alcoholic hepatic injury.