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Determination of anthocyanins in four Croatian cultivars of sour cherries (Prunus cerasus)
Authors:Valentina Šimunić  Spomenka Kovač  Dajana Gašo-Sokač  Werner Pfannhauser  Michael Murkovic
Affiliation:(1) Faculty of Food Technology, Department for Fundamental and Applied Chemistry, Kuhaccaroneva 18, P.O. Box 31 000, Osijek, Croatia;(2) Institute for Food Chemistry and Technology, Graz University of Technology, Petersgasse 12, 8010 Graz, Austria
Abstract:The anthocyanins were characterised and quantified in four cultivars of sour cherries by means of high-performance liquid chromatography and mass spectrometry. Column chromatography on XAD-2 Amberlite was the method used for isolation of anthocyanins. A diode-array detector was employed. For anthocyanins determination was chosen wavelength of 518 nm. Cyanidin-3-glucosylrutinoside and cyanidin-3-rutinoside were major anthocyanins. The total anthocyanin content in sour cherries, (expressed as cyanidin-3-glucosylrutinoside) varied from 2.7 to 28.0 mg/100 g of fresh weight, with the highest content being observed in Oblaccaroninska cultivar.
Keywords:Sour cherries (Prunus cerasus)  Anthocyanins  High-performance liquid chromatography  Isolation
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