Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line |
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| Authors: | Masamichi Kajita Masaki MagariKagefumi Todo Naoki KanayamaHitoshi Ohmori |
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| Affiliation: | Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Kita-Ku, Okayama 700-8530, Japan |
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| Abstract: | A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression. |
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| Keywords: | Chicken B cell line Point mutation Gene conversion Monoclonal antibody XRCC3 |
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