Evaluation of photo-induced cellular damage using photoautotrophic cultures of puk-bung hairy roots as a sensing tool |
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| Authors: | Motomu Nishioka Yoshihiro Ojima Yuki TsushimaMasahiro Matsumoto Masahito Taya |
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| Affiliation: | Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan |
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| Abstract: | In photoautotrophic cultures of pak-bung hairy roots, strong light irradiation (22 W/m2) increased the content of reactive oxygen species (ROS) in the cells, resulting in chlorophyll (Chl) degradation and DNA injury. The Chl degradation rate, RD, can be used as a parameter to measure the cellular damage caused by photo-induced stress. The presence of the antioxidants ascorbic acid, chlorogenic acid or catechin reduced the RD, while lowering the content of ROS and moderating the DNA injury. |
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| Keywords: | Plant cell culture Photoautotrophic puk-bung hairy roots Photo-induced damage Reactive oxygen species Antioxidants |
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