微波辅助顶空固相微萃取法快速分析黔产一年蓬不同部位挥发油化学成分

采用微波辅助顶空固相微萃取法(MAE-HS-SPME)快速分析一年蓬不同部位的挥发性化学成分,通过气相色谱-质谱法与Kovats色谱保留指数相结合进行定性定量分析,在一年蓬的茎、叶和花中分别鉴定出67、69和69种化合物,分别占挥发油总峰面积的92.71%、96.72%和96.16%,在茎中主要成分是β-榄香烯(5.22%),大根香叶烯D(41.80%)和(+)-β-蛇床烯(7.71%);叶中主要成分是3-甲基-2-环戊烯-2-醇-1-酮(6.43%),β-榄香烯(8.45%),大根香叶烯D(40.17%)和(E,E)-α-金合欢烯(5.80%);而在花中主要是3-甲基-2-环戊烯-2-醇-1-酮(4.20%),β-榄香烯(8.65%)和大根香叶烯D(31.20%);在茎、叶、花中各主要成分及其相对质量分数有差异。

Rapid Analysis of the Chemical Composition of Volatile Oils in Different Parts of Herba Erigerontis Annui of Guizhou Origin by Method of MAE-HS-SPME Followed by GC-MS

The volatile compounds were extracted from the different parts of Herba Erigerontis Annui by method of microwave-assisted headspace solid-phase micro-extraction(MAE-HS-SPME).Their constituents were identified and analyzed by method of gas chromatography-mass spectrometry combined with Kovats retention index.The results show that 67,69 and 69 kinds of volatile constituents were identified in the stem,the leaf and the flower of Herba Erigerontis Annui respectively accounting for 92.71%,96.72% and 96.16% of the total peak areas respectively.The main components in stem are β-elemene(5.22%),germacrene D(41.80%) and(+)-β-selinene(7.71%),while the main components in the leaves are 3-methyl-2-cyclopenten-2-ol-1-one(6.43%),β-elemene(8.45%),germacrene D(40.17%) and(E,E)-α-farnesene(5.80%).The main components in the flowers are 3-methyl-2-cyclopenten-2-ol-1-one(4.20%),β-elemene(8.65%) and germacrene D(31.20%).It was also observed that there is significant difference in the relative contents of the three parts.