固相萃取-反相高效液相色谱测定食品中大豆异黄酮含量的方法研究

该研究建立了一种固相萃取-反相高效液相色谱同时测定大豆和大豆制品中黄豆黄素、黄豆黄苷、大豆苷、大豆苷元、染料木 素、染料木苷、鸢尾黄酮苷和鹰嘴豆芽素A共8种异黄酮含量的方法。 采用体积分数为90%甲醇水溶液提取异黄酮，提取液用C18固相 萃取柱净化，采用C18色谱柱分离，柱温40 ℃，以乙腈和磷酸水溶液为流动相，流速1.0 mL/min，紫外检测器254 nm进行检测，其重复测 定结果的相对标准偏差（RSD）均＜5%，加标回收率为79.3%～102.5%。所建立的反相高效液相色谱法是一种高灵敏度、高准确度的 测定方法，适用范围广，对食品中大豆异黄酮的质量控制和合理有效利用提供了参考依据，具有一定的理论意义和应用价值。

Determination of soy isoflavones in food by solid-phase extraction and reversed-phase high performance liquid chromatography

A method for simultaneously determination of the contents of eight kinds of soy isoflavones including glycitein, glycitin, daidzin, daidzein, genistein, genistin, tectoridin, biochanin A in soy and soybean food by solid phase extraction-reversed phase high performance liquid chromatography (RP-HPLC) was established. After the isoflavones were extracted by 90% methanol aqueous solution, the extracted solution of sample was purified using C18 solid phase extraction column and separated by C18 chromatographic column, the column temperature was 40 ℃. Acetonitrile-phosphate so- lution was used as mobile phase with the flow rate of 1.0 ml/min, and the detection wavelength was 254 nm. The repetitive test results showed that the relative standard deviation (RSD) was less than 5%, and the recovery rate was in the range of 79.3%-102.5%. The established RP-HPLC method was good with high sensitivity and good accuracy, which had wide range of application. The results provided a technical reference for quality control, reasonable and effective utilization of soy isoflavones in food, and had certain theoretical significance and application value.