| 木糖浓度及补料发酵对树干毕赤酵母乙醇发酵的影响 |
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| 引用本文： | 吴仁智,陈东,黄俊,陆琦,芦志龙,陈英,陈小玲,黄日波. 木糖浓度及补料发酵对树干毕赤酵母乙醇发酵的影响[J]. 中国酿造, 2018, 37(12): 112. DOI: 10.11882/j.issn.0254-5071.2018.12.022 |
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| 作者姓名： | 吴仁智 陈东 黄俊 陆琦 芦志龙 陈英 陈小玲 黄日波 |
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| 作者单位： | （1.广西科学院 国家非粮生物质能源工程技术研究中心 非粮生物质酶解国家重点实验室 广西生物质产业化工程院广西生物炼制重点实验室，广西 南宁 530007；2.广西大学 生命科学与技术学院 亚热带农业生物资源保护与利用国家重点实验室，广西 南宁 530004） |
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| 基金项目： | 国家863项目（2013AA050701）；广西自然科学基金联合资助培育项目-面上项目（2018GXNSFAA138111）；广西重点研发计划（桂科AB16380024）；广西科技开发项目（桂科合14123001-6）；广西科学院基本科研业务费（12YJ25SW04）；广西科学院科研发展基金项目（2018YFJ402） |
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| 摘 要： | 探究不同浓度木糖及补料对树干毕赤酵母（Pichia stipitis）菌株1K-9发酵木糖产乙醇的影响，提高木糖产乙醇的发酵水平，为扩大规模发酵木糖产乙醇打下基础。结果表明，菌株1K-9先采用10%木糖进行乙醇发酵，36 h补加与10%木糖培养基等体积的20%木糖培养基继续发酵，发酵至108 h时菌数也达到了（12.16±0.07）×108个/mL，较未补料发酵时有所提高；发酵108 h时醪液中残留的木糖含量为（1.03±0.02）g/L，较未补料发酵时有所降低；乙醇含量达到了6.56%vol，较未补料时提高了1.85%vol。因此补料发酵是有效的。
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| 关 键 词： | 树干毕赤酵母 乙醇 木糖 补料发酵 |
Effect of xylose concentration and fed-batch fermentation on Pichia stipitis fermentation for ethanol production from xylose |
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| WU Renzhi,CHEN Dong,HUANG Jun,LU Qi,LU Zhilong,CHEN Ying,CHEN Xiaoling,HUANG Ribo. Effect of xylose concentration and fed-batch fermentation on Pichia stipitis fermentation for ethanol production from xylose[J]. China Brewing, 2018, 37(12): 112. DOI: 10.11882/j.issn.0254-5071.2018.12.022 |
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| Authors: | WU Renzhi CHEN Dong HUANG Jun LU Qi LU Zhilong CHEN Ying CHEN Xiaoling HUANG Ribo |
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| Affiliation: | (1.National Engineering Research Center for Non-food Biorefinery, State Key Laboratory of Non-food Biomass Enzyme Technology, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China; 2.College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China) |
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| Abstract: | The effect of different concentration of xylose and fed-batch fermentation on the ethanol production of Pichia stipitis 1K-9 from xylose was investigated, to improve the level of ethanol production and to lay a foundation for increasing the ethanol production from xylose fermentation. The results indicated that strain 1K-9 was supplemented with 10% xylose for ethanol fermentation, and was fed-batch with 20% xylose medium (the same volume of 10% xylose medium) after 36 h fermentation. The microbial count was (12.16±0.07)×108 CFU/ml at 108 h, which was increased than that before fed- batch fermentation. The residual xylose content in the mash was (1.03±0.02) g/L, which was decreased than that before fed-batch fermentation. The ethanol content reached 6.56%vol, which was 1.85%vol higher than that before fed-batch fermentation. Therefore, supplementary fermentation was effective. |
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| Keywords: | Pichia stipitis ethanol xylose fed-batch fermentation |
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