一株海洋链霉菌发酵条件的优化及其抑菌活性物质的研究

灰略红链霉菌（Streptomyces griseorubens）F8分离于黄海海岸线沉淀物，通过单因素试验优化其发酵条件，并对其代谢产物及抑菌活性进行研究。结果表明，最佳发酵条件为培养时间11 d、接种量8%、培养温度30 ℃、初始pH值8.0。菌株F8所产抑菌物质对金黄色葡萄球菌（Staphylococcus aureus），变形杆菌（Proteus）和产气杆菌（Aerobacter aerogenes）具有抑制作用，抑菌圈直径分别为23 mm、34 mm、44 mm，最小抑菌浓度（MIC）分别为100 μg/mL、30 μg/mL、20 μg/mL。菌株F8所产抑菌活性物质的酸碱、热、光照以及遗传稳定性均较好。

Optimization of fermentation conditions of a marine Streptomyces and its antibacterial activity substances

Streptomyces griseorubens F8 was isolated from the sediment in the Yellow Sea coastline. The fermentation conditions were optimized by single factor experiments, and its metabolites and antibacterial activities were studied. The results showed that the optimal fermentation conditions were determined as follows: culture time 11 d, inoculum 8%, temperature 30 ℃, and the initial pH value of medium 8.0. The antibacterial substances produced by strain F8 had inhibiting effects on Staphylococcus aureus, Proteus and Aerobacter aerogenes, and the inhibition zone diameter were 23 mm, 34 mm, and 44 mm, respectively; the minimum inhibitory concentration (MIC) were 100 μg/ml, 30 μg/ml, and 20 μg/ml, respectively. The acid-base, thermal, light and genetic stability of antibacterial substances produced by strain F8 were good.