| 植物乳杆菌亚油酸异构酶的分离纯化及其性质研究 |
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| 引用本文： | 苗士达,张中义,刘萍,柴秋儿,胡锦荣,孙君社. 植物乳杆菌亚油酸异构酶的分离纯化及其性质研究[J]. 食品与发酵工业, 2005, 31(3): 12-15 |
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| 作者姓名： | 苗士达 张中义 刘萍 柴秋儿 胡锦荣 孙君社 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 郑州轻工业学院食品科学与生物工程系,郑州,450002 |
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| 基金项目： | 国家自然科学基金 (No .2 0 3760 84) |
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| 摘 要： | 经硫酸铵分级沉淀、阴离子交换层析和凝胶过滤,由植物乳杆菌(LactobacillusplantarumL 2 9)分离纯化得到亚油酸异构酶,分子量为4 3ku。对其酶学性质进行研究,结果表明,温度37℃、pH 6 0时酶活性较高;Co2 + 、Fe2 + 可提高酶的活性,Cu2 + 、Zn2 + 则对酶活力有抑制作用;该酶作用于亚油酸的Km=2 5 3×10 -5mol/L ,Vmax=2 5 7×10 -8mol/ (min·mg)。
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| 关 键 词： | 植物乳杆菌 亚油酸异构酶 共轭亚油酸 |
| 修稿时间： | 2004-10-11 |
Purification and Characterization of a Linoleic Acid Isomerase from a Lactobacillus plantarum Bateria |
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| Miao Shida,Zhang Zhongy,Liu Ping,Chai Qiuer,Hu Jinrong,Sun Junshe. Purification and Characterization of a Linoleic Acid Isomerase from a Lactobacillus plantarum Bateria[J]. Food and Fermentation Industries, 2005, 31(3): 12-15 |
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| Authors: | Miao Shida Zhang Zhongy Liu Ping Chai Qiuer Hu Jinrong Sun Junshe |
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| Affiliation: | Miao Shida 1 Zhang Zhongy 1,2 Liu Ping 1 Chai Qiuer 1 Hu Jinrong 1 Sun Junshe 1 1 |
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| Abstract: | A linoleic acid isomerase was purified from Lactobacillus plantarum L-29 by ammonium sulfate precipitation,DEAE Sepharose fast flow and Sephadex G-100 column chromatography. The molecular weight of the enzyme was about 43ku. The enzyme exhibited higher activity when temperature and pH were 30℃,6.0 respectively. Co 2+ ,Fe 2+ improved the activity of the enzyme, and Cu 2+ ,Zn 2+ instead decreased the activity of the enzyme. Mcihael constants of the enzyme were K m=2.53×10 -5 ?mol/L and V max =2.57×10 -8 ?mol/(min·mg) when substrate was linoleic acid. |
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| Keywords: | Lactobacillus plantarum linoleic acid isomerase conjugated linoleic acid |
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