| 一株抗铅细菌的筛选及鉴定 |
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| 引用本文： | 冀伟,刘瑞,常亮,于源华.一株抗铅细菌的筛选及鉴定[J].长春理工大学学报,2014(6):147-150. |
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| 作者姓名： | 冀伟 刘瑞 常亮 于源华 |
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| 作者单位： | 1. 长春理工大学 生命科学技术学院,长春,130022
2. 长春医学高等专科学校,长春,130013 |
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| 摘 要： | 重金属铅对环境和人类危害极大,有些微生物具有耐受性,可用于治理环境中的铅。从电镀厂附近的土壤中分离、筛选出一株可抗Pb2＋浓度达2000mg/L的菌株FP 2000,该菌经过革兰氏染色,在显微镜下观察确定为短棒状革兰氏阴性菌,最适合的生长条件为：温度34-40℃,p H值4-6。并对该菌株16S r DNA进行测序,分析测序结果后鉴定了该菌种为克雷伯氏菌。为进一步研究微生物抗铅机理奠定基础。
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| 关 键 词： | 抗铅 微生物 筛选及鉴定 |
Screening and Determination of a Lead-tolerant Bacterial Strain |
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| JI Wei,LIU Rui,CHANG Liang,YU Yuanhua.Screening and Determination of a Lead-tolerant Bacterial Strain[J].Journal of Changchun University of Science and Technology,2014(6):147-150. |
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| Authors: | JI Wei LIU Rui CHANG Liang YU Yuanhua |
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| Affiliation: | JI Wei, LIU Rui , CHANG Liang, YU Yuanhua ( 1. School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022; 2. Changchun Medical College, Changchun 130013) |
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| Abstract: | From soil of an electroplate factory,a bacterial strain with high efficiency of resisting lead was found,which can resist a high concentration（2000mg/L） Pb2＋,and was named as FP2000 in this study.By the comparison between growth conditions and lead resistance,the optimum resistance conditions were determined,which temperature was 37-40℃ and p H was 5.0-7.0.Based on the result of 16 S r DNA sequences,this strain was identified as Klebsiella.The foundations is laid for the further study on mechanism of microbial resistance to lead. |
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| Keywords: | lead-resistance microbe screening and determination |
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