| 腐竹生产过程中豆浆游离氨基酸的HPLC法测定 |
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| 引用本文： | 陈悦娇,白卫东,黄桂颖,马应丹,张海燕.腐竹生产过程中豆浆游离氨基酸的HPLC法测定[J].食品与发酵工业,2008,34(7). |
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| 作者姓名： | 陈悦娇 白卫东 黄桂颖 马应丹 张海燕 |
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| 作者单位： | 仲恺农业技术学院轻工食品学院,广东,广州,510225 |
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| 摘 要： | 建立一种测定豆浆中游离氨基酸的反相高效液相色谱法。优化条件为:Eclipse XDB—C_(18)反相柱(150mm×4.6mm,5μm);流动相:甲醇和0.05mol/L醋酸钠的体积比线性梯度(T,B%)=0,0%;20,20%;50,90%,流速:1.0mL/min,检测波长:338nm。在此条件下得到各游离氨基酸的直线回归方程,相关系数都在0.9900以上。豆浆煮制过程中游高氨基酸含量逐步增加,回收率在90.86%～106.05%之间。
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| 关 键 词： | HPLC 豆浆 游离氨基酸 测定 |
The Detection of Free Amino Acids in Soybean Milk by Revered Phase HPLC |
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| Chen Yuejiao,Bai Weidong,Ma Yingdan,Huang Guiying,Zhang Haiyan.The Detection of Free Amino Acids in Soybean Milk by Revered Phase HPLC[J].Food and Fermentation Industries,2008,34(7). |
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| Authors: | Chen Yuejiao Bai Weidong Ma Yingdan Huang Guiying Zhang Haiyan |
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| Abstract: | In this paper,a method of reverse high performance liquid chromatography for determinating the free amino acids in soybean milk was established.The optimized chromatographic condition were as fol- lowed:Eclipse XDB—C18 stainless column (150mm×4.6mm,5μm) was used.The mobile phase was as fol- lows:V (methanol):V(0.05mol/LNaAC (T,B%) =0,0%.20,20%.50,90%,and the flow rate was 1.0 mL/ min.It was measured by an ultraviolet detector under 338 nm.The coefficients of linear equations of 18 free amino acid were all above 0.9900.The free amino acids in soybean milk increased with the increasing of the time of boiling.The recoveries were 90.86%～106.05%. |
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| Keywords: | HPLC |
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