酵母源金属硫蛋白对慢性铅中毒小鼠排铅及肝脏保护作用

探讨具有自主知识产权酵母源金属硫蛋白(MT)对慢性铅中毒小鼠的排铅及肝损伤保护作用。以二巯基丁二酸(DMSA)与动物源金属硫蛋白(兔肝Zn-MT)为对照,醋酸铅溶液致慢性铅中毒小鼠连续灌胃给予MT 35 d后,测定小鼠体重、血铅、部分血常规及肝功能指标水平,观察小鼠肝组织病变程度。与模型组比较,MT处理组小鼠体重无显著变化(P0.05);高剂量MT处理组小鼠血铅含量显著下降(P0.05),白细胞、红细胞平均体积及血红蛋白水平显著升高(P0.05),但各中毒组小鼠粒细胞计数水平无显著差异性(P0.05);各给药组小鼠GPT水平均显著下降(P0.05),同时高剂量MT处理组小鼠GOT水平均显著下降(P0.05),小鼠肝组织病变程度明显改善,其改善程度优于DMSA组。两种酵母源金属硫蛋白对慢性铅中毒小鼠均具有明显的排铅及肝脏保护作用,作用效果类似于动物源金属硫蛋白。

Lead-expelling and Liver-protecting Effects of Yeast Metallothionein on Mice with Chronic Lead Poisoning

The aim of this study was to investigate the effect of yeast metallothionein (MT) with known lead-expelling and liver-protecting properties on mice in which, chronic lead poisoning was induced by lead acetate solution gavage for 35 days. Dimercaptosuccinic acid (DMSA) and animal MT (rabbit liver Zn-MT) were used as controls and MT was administered to the mice. Subsequently, the weight, blood lead levels, and liver function indices were measured and a routine blood tests were conducted to observe the severity of pathological changes in the liver tissue. Compared with that of the control group, the weight of MT-treated groups was not significantly different. The blood lead level in high-dose MT-treated group was significantly decreased, while the levels of white blood cells (WBC), mean corpuscular volume (MCV), and hemoglobin (HGB) were significantly increased (p < 0.05). However, there were no significant differences in granulocyte (GR) counts between all groups (p > 0.05). The levels of glutamate pyruvate transaminase (GPT) in all MT-treated groups were significantly decreased (p <0.05). Meanwhile, the levels of glutamate oxaloacetate transaminase (GOT) in the high-dose MT-treated group were significantly decreased (p <0.05), pathological changes in liver tissue were ameliorated, and the degree of improvement was higher than that of DMSA group. Two types of yeast metallothionein exhibited lead-expelling and liver-protecting effects on mice with chronic lead poisoning, similar to animal metallothionein.