自噬在肾间质纤维化小鼠中的表达趋势

目的: 观察肾间质纤维化单侧输尿管梗阻模型（UUO）术后不同时间点自噬的表达趋势。方法: 32只清洁级健康雄性ICR小鼠随机分组：假手术（Sham）组、UUO模型（UUO）组，分别在 UUO术后 3、7、14 d 处死小鼠，假手术组7 d处死，每组小鼠8只。留取血清检测血肌酐（Scr）、尿素氮（BUN），肾组织行HE和Masson染色，观察光镜病理结果，电镜超微结构观察自噬体的形成，Westernblot检测肾组织LC3蛋白和P62蛋白的表达。结果: UUO术后梗阻侧肾脏皮质变薄，小管萎缩，间质纤维化，单个核细胞浸润，肾小球无明显病变，病理变化以UUO术后14 d最突出。与Sham组相比，LC3-Ⅱ蛋白含量从3 d开始逐渐增高，7 d时达峰值，14 d时下降；而P62与LC3-Ⅱ相反，3 d开始下降，7 d达最低值，14 d又逐渐恢复，表明自噬在7 d这个时间点表达最显著。电镜显示UUO术后7 d出现自噬小体和吞噬泡现象。结论: UUO术后自噬的表达有一定的时间依赖性，在7 d时表达最显著，自噬与病理严重程度不平行。

Expression trend of autophagy in mice with renal interstitial fibrosis

AIM: To observe the expression trend of autophagy at different time points after UUO surgery. METHODS: Thirty-two clean healthy male ICR mice were randomized into Sham operation (Sham) group and UUO model (UUO) group, and mice were sacrificed at 3, 7 and 14 d after UUO surgery, respectively, 8 mice for each group. Serum was collected to detect Scr, BUN, HE and Masson staining ways were applied to the renal tissue, and the formation of autophagosomes was observed by electron microscopy and the expression of LC3 protein and P62 protein in renal tissue was detected by Westernblot. RESULTS:The renal cortex thinned, tubule atrophy, interstitial fibrosis, single nuclear cell infiltration, no obvious glomerular lesions were found in the obstructed side of UUO postoperatively, and the pathological changes were most prominent on 14 d after UUO surgery. Compared with the Sham group, LC3-Ⅱbegan from 3 d, peaked on 7 d, down on 14 d; P62 was different, 3 d began to decline, 7 d reached the lowest, 14 d recovered gradually, which showed that autophagy was most significant at 7 d. Electron microscopy showed that autophagosome and phagocytic vacuoles appeared 7 days after UUO surgery.CONCLUSION: The expression of autophagy after UUO surgery had a certain time dependence, which was most significant at 7 d, and the severity of autophagy was not parallel to the pathological severity.