黄芪甲苷通过SDF-1/CXCR4信号通路诱导人肾腺癌细胞的凋亡

目的:观察黄芪甲苷对人肾腺癌细胞ACHN增殖、凋亡的作用及其对SDF-1/CXCR4信号通路活化的影响。方法:采用20、60、120 μmol/L黄芪甲苷干预ACHN细胞后，CCK-8分析黄芪甲苷对细胞增殖抑制率的影响，流式细胞仪检测黄芪甲苷对细胞凋亡率的影响，Western Blot方法分析黄芪甲苷对细胞中凋亡相关蛋白Csapase-8、Bax、Bcl-2及SDF-1/CXCR4信号通路相关蛋白表达的影响。结果:黄芪甲苷干预ACHN细胞后，细胞增殖抑制率明显升高，表现出时间和剂量依赖性（P<0.05）；黄芪甲苷干预组ACHN细胞凋亡率也显著升高（P<0.05）；药物处理组中促凋亡蛋白Csapase-8、Bax表达显著升高，而抗凋亡蛋白Bcl-2及SDF-1、CXCR4蛋白表达水平显著降低，表现出剂量依赖性（P<0.05）。结论:黄芪甲苷抑制人肾腺癌细胞株ACHN增殖、诱导其凋亡的作用机制可能与降低SDF-1/CXCR4信号通路活化相关。

Effect of astragaloside on the apoptosis of human renal carcinoma cell (ACHN) via SDF-1/CXCR4 signaling pathway

AIM: To observe the effect of astragaloside on the proliferation, apoptosis of human renal carcinoma cell (ACHN) and the activation of SDF-1/CXCR4 signaling pathway. METHODS: ACHN cells were co-cultured with 20, 60 and 120 μmol/L of astragaloside. CCK-8 assay was used to detect the effect of astragaloside on proliferation inhibitory rates of ACHN cells. The apoptotic rates of ACHN cells were analyzed using flow cytometer. Western Blot was used to determine the expression levels of Csapase-8, Bax, Bcl-2, SDF-1 and CXCR4 proteins in ACHN cells. RESULTS:After treatment with 20, 60 and 120 μmol/L of astragaloside, the proliferation inhibitory rates of ACHN cells were significantly increased with time- and dose-dependent manner (P<0.05). The apoptotic rates of ACHN cells in astragaloside groups were significantly increased compared with control group (P<0.05). The expression levels of Csapase-8 and Bax in astragaloside groups were significantly increased, and the expression levels of Bcl-2, SDF-1 and CXCR4 were significantly decreased compared with control group (P<0.05). CONCLUSION: Astragaloside can inhibit the proliferation of ACHN cells and induce the apoptosis of ACHN cells, and its mechanism was associated with the inhibition of SDF-1/CXCR4 cascade.