| lncFOXC1表达增加促进肝癌发生 |
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| 引用本文： | 周玉玲,刘激扬,姚红伟,徐玉芬,张 婷,邹卓林,潘 欢. lncFOXC1表达增加促进肝癌发生[J]. 金属学报, 2019, 24(1): 27-31. DOI: 10.12092/j.issn.1009-2501.2019.01.005 |
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| 作者姓名： | 周玉玲 刘激扬 姚红伟 徐玉芬 张 婷 邹卓林 潘 欢 |
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| 作者单位： | 1.中国人民武装警察部队药品仪器检验所，北京 102613； ;2.嘉兴市第一医院，嘉兴 314000，浙江；;3.北京大学医学部病原微生物学系，北京 100191 |
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| 基金项目： | 浙江省医药卫生科技计划项目（2016KYB290）；嘉兴市科技计划项目（2018AY32011）；嘉兴市第一医院院级课题（2016-YA-14） |
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| 摘 要： | 目的: 探讨长链非编码RNA lncFOXC1在肝癌组织中的表达以及在肝癌干细胞生长中的作用。方法: 收集34例手术切除的肝癌组织和癌旁组织样本，实时荧光定量PCR检测lncFOXC1的表达。同时检测lncFOXC1在肝癌干细胞小球中的表达。建立lncFOXC1低表达的稳定细胞系，通过干细胞小球形成和小鼠皮下成瘤实验观察lncFOXC1对肿瘤形成的作用。 结果: lncFOXC1在肝癌组织和干细胞小球中显著高表达（P<0.05）。lncFOXC1敲减后显著抑制了肝癌干细胞小球形成和小鼠皮下肿瘤的生长。结论: lncFOXC1在肝癌组织中高表达，调控肝癌干细胞生长。
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| 关 键 词： | lncFOXC1 肝癌 肿瘤干细胞 |
| 收稿时间： | 2018-09-03 |
| 修稿时间： | 2018-09-29 |
Increased expression of lncFOXC1 promotes liver cancer formation |
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| ZHOU Yuling,LIU Jiyang,YAO Hongwei,XU Yufen,ZHANG Ting,ZOU Zhuolin,PAN Huan. Increased expression of lncFOXC1 promotes liver cancer formation[J]. Acta Metallurgica Sinica, 2019, 24(1): 27-31. DOI: 10.12092/j.issn.1009-2501.2019.01.005 |
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| Authors: | ZHOU Yuling LIU Jiyang YAO Hongwei XU Yufen ZHANG Ting ZOU Zhuolin PAN Huan |
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| Affiliation: | 1. Institute of Drug Control and Instrument Maintenance of Chinese People's Armed Police Force, Beijing 102613, China;2. The First Hospital of Jiaxing, Jiaxing 314000, Zhejiang，China;3.Department of Microbiology & Infectious Disease Center, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China |
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| Abstract: | AIM: To investigate the expression of long noncoding RNA lncFOXC1 in liver cancer and the role in liver cancer formation. METHODS: A total of 34 liver cancer and peri-tumor tissues were collected. Expression of lncFOXC1 was detected by qRT-PCR in liver cancer and peri-tumor tissues, as well as liver cancer cell spheres. LncFOXC1 deficiency cell lines were constructed by shRNA. Then, its roles in sphere formation and in vivo tumor growth assays were studied. RESULTS: lncFOXC1 was significantly highly expressed in liver cancer tissues and liver cancer cell sphere. LncFOXC1 deficiency significantly reduced sphere formation and tumor growth. CONCLUSION: LncFOXC1 is highly expressed in liver cancer tissues, and can regulate liver cancer stem cell growth. |
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| Keywords: | lncFOXC1 liver cancer cancer stem cell |
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